A Tacoma native, Trufant was selected out of Washington State by Seattle in the first round of the 2003 NFL Draft and spent all 10 seasons of his NFL career with his hometown team. As a rookie, he recorded two interceptions, one fumble recovery, and 83 total tackles on his way to being named to the 2003 NFL All-Rookie Team. In 2006, the team nominated him Seahawks Man of the Year. The following season, he recorded seven interceptions, including three in a single game against Arizona which is tied for the most in franchise history, and was named to the 2007 Pro Bowl. Trufant served as the defensive team captain in 2011 and his 21 career interceptions is seventh-most in franchise history. In April 2014, after having spent the 2013 offseason with the Jacksonville Jaguars, Trufant signed a one-day contract and retired as a Seahawk.